Adeno-associated viral vector gene transfer into leptomeningeal xenografts.

Publication Type	Academic Article
Authors	Rosenfeld M, Bergman I, Schramm L, Griffin J, Kaplitt M, Meneses P
Journal	J Neurooncol
Volume	34
Issue	2
Pagination	139-44
Date Published	09/01/1997
ISSN	0167-594X
Keywords	Cerebellar Neoplasms, Gene Transfer Techniques, Genetic Therapy, Medulloblastoma, Meningeal Neoplasms, Subarachnoid Space
Abstract	Leptomeningeal carcinomatosis is a painful and debilitating complication of cancer. Indwelling reservoirs provide continuous assess to the subarachnoid space, making leptomeningeal cancer potentially amenable to gene therapy. Adeno-associated virus (AAV) is a defective virus not associated with any human disease. We used an AAV vector to transduce medulloblastoma (DAOY) cells in a nude rat model of leptomeningeal disease. After intraventricular injection of vector carrying the bacterial lacZ gene, beta-galactosidase positive cells were found in the implanted tumor and in ependymal and subependymal cells but not in underlying normal brain parenchyma. No evidence of virally-mediated toxicity was noted in the animals. The results of this pilot study demonstrate that AAV vectors may be used to transfer and express foreign genes in established leptomeningeal tumors.
DOI	10.1023/a:1005702228721
PubMed ID	9210060