Adeno-associated viral vector gene transfer into leptomeningeal xenografts.
| Publication Type | Academic Article |
| Authors | Rosenfeld M, Bergman I, Schramm L, Griffin J, Kaplitt M, Meneses P |
| Journal | J Neurooncol |
| Volume | 34 |
| Issue | 2 |
| Pagination | 139-44 |
| Date Published | 09/01/1997 |
| ISSN | 0167-594X |
| Keywords | Cerebellar Neoplasms, Gene Transfer Techniques, Genetic Therapy, Medulloblastoma, Meningeal Neoplasms, Subarachnoid Space |
| Abstract | Leptomeningeal carcinomatosis is a painful and debilitating complication of cancer. Indwelling reservoirs provide continuous assess to the subarachnoid space, making leptomeningeal cancer potentially amenable to gene therapy. Adeno-associated virus (AAV) is a defective virus not associated with any human disease. We used an AAV vector to transduce medulloblastoma (DAOY) cells in a nude rat model of leptomeningeal disease. After intraventricular injection of vector carrying the bacterial lacZ gene, beta-galactosidase positive cells were found in the implanted tumor and in ependymal and subependymal cells but not in underlying normal brain parenchyma. No evidence of virally-mediated toxicity was noted in the animals. The results of this pilot study demonstrate that AAV vectors may be used to transfer and express foreign genes in established leptomeningeal tumors. |
| DOI | 10.1023/a:1005702228721 |
| PubMed ID | 9210060 |