Detection of messenger RNA and low-abundance heteronuclear RNA with single-stranded DNA probes produced by amplified primer extension labeling.

Publication Type	Academic Article
Authors	Brooks P, Kaplitt M, Kleopoulos S, Funabashi T, Mobbs C, Pfaff D
Journal	J Histochem Cytochem
Volume	41
Issue	12
Pagination	1761-6
Date Published	12/01/1993
ISSN	0022-1554
Keywords	DNA Probes, DNA, Single-Stranded, In Situ Hybridization, RNA, Heterogeneous Nuclear, RNA, Messenger
Abstract	We describe a procedure for detection of low-abundance cellular RNAs by in situ hybridization histochemistry, using single-stranded DNA probes produced by amplified primer extension labeling with Taq polymerase. We have used this approach to detect a number of high- and low-abundance RNA species and have found it to be a simple and reproducible method of obtaining sensitive probes for in situ hybridization studies. For example, DNA probes generated by amplified primer extension labeling can detect low-abundance heteronuclear RNAs in individual neurons. Since this procedure does not involve recombinant DNA technology or microbiological facilities, it should prove useful to a wide variety of investigators studying the regulation of gene expression at the cellular level.
DOI	10.1177/41.12.8245424
PubMed ID	8245424