Publication Type Academic Article
Authors Brooks P, Kaplitt M, Kleopoulos S, Funabashi T, Mobbs C, Pfaff D
Journal J Histochem Cytochem
Volume 41
Issue 12
Pagination 1761-6
Date Published 12/01/1993
ISSN 0022-1554
Keywords DNA Probes, DNA, Single-Stranded, In Situ Hybridization, RNA, Heterogeneous Nuclear, RNA, Messenger
Abstract We describe a procedure for detection of low-abundance cellular RNAs by in situ hybridization histochemistry, using single-stranded DNA probes produced by amplified primer extension labeling with Taq polymerase. We have used this approach to detect a number of high- and low-abundance RNA species and have found it to be a simple and reproducible method of obtaining sensitive probes for in situ hybridization studies. For example, DNA probes generated by amplified primer extension labeling can detect low-abundance heteronuclear RNAs in individual neurons. Since this procedure does not involve recombinant DNA technology or microbiological facilities, it should prove useful to a wide variety of investigators studying the regulation of gene expression at the cellular level.
DOI 10.1177/41.12.8245424
PubMed ID 8245424
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