Detection of messenger RNA and low-abundance heteronuclear RNA with single-stranded DNA probes produced by amplified primer extension labeling.
| Publication Type | Academic Article |
| Authors | Brooks P, Kaplitt M, Kleopoulos S, Funabashi T, Mobbs C, Pfaff D |
| Journal | J Histochem Cytochem |
| Volume | 41 |
| Issue | 12 |
| Pagination | 1761-6 |
| Date Published | 12/01/1993 |
| ISSN | 0022-1554 |
| Keywords | DNA Probes, DNA, Single-Stranded, In Situ Hybridization, RNA, Heterogeneous Nuclear, RNA, Messenger |
| Abstract | We describe a procedure for detection of low-abundance cellular RNAs by in situ hybridization histochemistry, using single-stranded DNA probes produced by amplified primer extension labeling with Taq polymerase. We have used this approach to detect a number of high- and low-abundance RNA species and have found it to be a simple and reproducible method of obtaining sensitive probes for in situ hybridization studies. For example, DNA probes generated by amplified primer extension labeling can detect low-abundance heteronuclear RNAs in individual neurons. Since this procedure does not involve recombinant DNA technology or microbiological facilities, it should prove useful to a wide variety of investigators studying the regulation of gene expression at the cellular level. |
| DOI | 10.1177/41.12.8245424 |
| PubMed ID | 8245424 |