Publication Type Academic Article
Authors Dimov A, Gillen K, Nguyen T, Kang J, Sharma R, Pitt D, Gauthier S, Wang Y
Journal Tomography
Volume 8
Issue 3
Pagination 1544-1551
Date Published 06/14/2022
ISSN 2379-139X
Keywords Multiple Sclerosis, White Matter
Abstract Quantitative susceptibility mapping (QSM) facilitates mapping of the bulk magnetic susceptibility of tissue from the phase of complex gradient echo (GRE) MRI data. QSM phase processing combined with an R2* model of magnitude of multiecho gradient echo data (R2*QSM) allows separation of dia- and para-magnetic components (e.g., myelin and iron) that contribute constructively to R2* value but destructively to the QSM value of a voxel. This R2*QSM technique is validated against quantitative histology—optical density of myelin basic protein and Perls’ iron histological stains of rim and core of 10 ex vivo multiple sclerosis lesions, as well as neighboring normal appearing white matter. We found that R2*QSM source maps are in good qualitative agreement with histology, e.g., showing increased iron concentration at the edge of the rim+ lesions and myelin loss in the lesions’ core. Furthermore, our results indicate statistically significant correlation between paramagnetic and diamagnetic tissue components estimated with R2*QSM and optical densities of Perls’ and MPB stains. These findings provide direct support for the use of R2*QSM magnetic source separation based solely on GRE complex data to characterize MS lesion composition.
DOI 10.3390/tomography8030127
PubMed ID 35736875
PubMed Central ID PMC9228115
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