Publication Type Academic Article
Authors Apostolova L, Zarow C, Biado K, Hurtz S, Boccardi M, Somme J, Honarpisheh H, Blanken A, Brook J, Tung S, Lo D, Ng D, Alger J, Vinters H, Bocchetta M, Duvernoy H, Jack C, Frisoni G
Journal Alzheimers Dement
Volume 11
Issue 2
Pagination 139-50
Date Published 01/22/2015
ISSN 1552-5279
Keywords Alzheimer Disease, Hippocampus, Image Processing, Computer-Assisted, Magnetic Resonance Imaging
Abstract OBJECTIVE: The pathologic validation of European Alzheimer's Disease Consortium Alzheimer's Disease Neuroimaging Initiative Center Harmonized Hippocampal Segmentation Protocol (HarP). METHODS: Temporal lobes of nine Alzheimer's disease (AD) and seven cognitively normal subjects were scanned post-mortem at 7 Tesla. Hippocampal volumes were obtained with HarP. Six-micrometer-thick hippocampal slices were stained for amyloid beta (Aβ), tau, and cresyl violet. Hippocampal subfields were manually traced. Neuronal counts, Aβ, and tau burden for each hippocampal subfield were obtained. RESULTS: We found significant correlations between hippocampal volume and Braak and Braak staging (ρ = -0.75, P = .001), tau (ρ = -0.53, P = .034), Aβ burden (ρ = -0.61, P = .012), and neuronal count (ρ = 0.77, P < .001). Exploratory subfield-wise significant associations were found for Aβ in Cornu Ammonis (CA)1 (ρ = -0.58, P = .019) and subiculum (ρ = -0.75, P = .001), tau in CA2 (ρ = -0.59, P = .016), and CA3 (ρ = -0.5, P = .047), and neuronal count in CA1 (ρ = 0.55, P = .028), CA3 (ρ = 0.65, P = .006), and CA4 (ρ = 0.76, P = .001). CONCLUSIONS: The observed associations provide pathological confirmation of hippocampal morphometry as a valid biomarker for AD and pathologic validation of HarP.
DOI 10.1016/j.jalz.2015.01.001
PubMed ID 25620800
PubMed Central ID PMC4348340
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