Reproducibility of single- and multi-voxel 1H MRS measurements of intramyocellular lipid in overweight and lean subjects under conditions of controlled dietary calorie and fat intake.
| Publication Type | Academic Article |
| Authors | Shen W, Mao X, Wolper C, Heshka S, Dashnaw S, Hirsch J, Heymsfield S, Shungu D |
| Journal | NMR Biomed |
| Volume | 21 |
| Issue | 5 |
| Pagination | 498-506 |
| Date Published | 06/01/2008 |
| ISSN | 0952-3480 |
| Keywords | Lipids, Magnetic Resonance Imaging, Magnetic Resonance Spectroscopy, Muscle Fibers, Skeletal, Overweight |
| Abstract | The reproducibility of repeated single-voxel 1H MRS (SV-MRS) and spectroscopic imaging (MRSI) measurements of intramyocellular lipid (IMCL) in the tibialis anterior muscle of five lean and five overweight female Caucasians, during 7 days of controlled dietary fat and calorie intake, was assessed at 1.5 T. Duplicate measures of IMCL relative to total muscle creatine (IMCL/tCr) obtained 3 days apart by both SV-MRS and MRSI correlated well (r = 0.65 and r = 0.95, respectively, P < 0.05). The coefficients of variation for repeated measures of IMCL/tCr by SV-MRS and MRSI were 24.4% and 10.7%, respectively. IMCL/tCr measured by MRSI was higher in overweight subjects than in lean subjects (8.3 +/- 3.8 vs 4.3 +/- 2.4, P < 0.05). Although both methods achieved good reproducibility in measuring IMCL in vivo, MRSI was found to offer greater flexibility and reliability, and higher sensitivity to IMCL differences, whereas SV-MRS was advantageous with respect to shorter scan time and ease of implementation. |
| DOI | 10.1002/nbm.1218 |
| PubMed ID | 17955571 |
| PubMed Central ID | PMC2892914 |