Resolution enhanced NMR spectroscopy in biological systems via magnetic susceptibility matched sample immersion chambers.

Publication Type	Academic Article
Authors	Balloon D, Mahmood U, Jakubowski A, Koutcher J
Journal	Magn Reson Med
Volume	30
Issue	6
Pagination	754-8
Date Published	12/01/1993
ISSN	0740-3194
Keywords	Magnetic Resonance Spectroscopy
Abstract	A technique is described which reduced the magnetic susceptibility induced line broadening in NMR spectra obtained from three biological systems at 4.7 Tesla. Proton spectra from a sealed suspension of HL60 leukemic myeloblasts yielded minimum linewidths of 1.3 Hz at 200 MHz (0.0065 ppm) after 10 min of automated shimming. 31P spectra from an in vivo murine MCa mammary carcinoma yielded a well-resolved phosphorylcholine resonance without proton decoupling and with the resistive shim coil currents set to zero. 31P spectra from a perfused suspension of RIF-1 fibrosarcoma cells exhibited a gamma-nucleoside triphosphate resonance which was resolved into purine and pyrimidine components.
DOI	10.1002/mrm.1910300615
PubMed ID	8139459